Current Protocols Essential Laboratory Techniques

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory.  From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher.  Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Foreword Preface  Contributors List Conversion Factors Flow Chart: Application of Techniques Emily Wiley, Claremont Colleges Chapter 1 Volume/Weight Measurement Unit 1.1 Volume Measurement Andrew Zanella and Tom Davis, Claremont Colleges Unit 1.2 Weight Measurement Michael Guzy, Ohaus, Corp. Chapter 2 Concentration Measurement Unit 2.1 Spectrophotometry Rob Morris, Ocean Optics, Inc. Unit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy Sean R. Gallagher, UVP, LLC Chapter 3 Reagent Preparation Unit 3.1 Theoretical Discussion Deb Chakravarti, Keck Graduate Institute Unit 3.2 Practical Discussion Deb Chakravarti, Keck Graduate Institute Unit 3.3 Recipes In-House Chapter 4 Cell Culture Techniques Unit 4.1 Aseptic Technique Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine Unit 4.2 Culture of Escherichia coli and Related Bacteria Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine Chapter 5 Sample Preparation Unit 5.1 Centrifugation and Cell Fractionation In-House Unit 5.2 Purification and Concentration of Nucleic Acids Dennis H. Dowhan, University of Queensland Chapter 6 Chromatography Unit 6.1 Overview of Chromatography Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences Unit 6.2 Column Chromatography Deb Chakravarti, Keck Graduate Institute Chapter 7 Electrophoresis Unit 7.1 Overview of Electrophoresis Sean R. Gallagherr, UVP, LLC Unit 7.2 Agarose Gel Electrophoresis Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College Unit 7.3 SDS-PAGE Sean Gallagher, UVP, LLC Unit 7.4 Staining of Gels Sean Gallagher, UVP, LLC Chapter 8 Blotting Unit 8.1 Overview of Blotting Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute Unit 8.2 Nucleic Acid Blotting: Southern and Northern Laura L. Mays Hoopes, Pomona College Unit 8.3 Protein Blotting: Immunoblotting Sean Gallagher, UVP, LLC Unit 8.4 Labeling DNA and Probe Preparation Karl A. Haushalter, Harvey Mudd College Chapter 9 Microscopy Unit 9.1 Conventional Light Microscopy Eric S. Cole, St. Olaf College Unit 9.2 Immunofluorescence Microscopy David J. Asai, Harvey Mudd College Chapter 10 Enzymatic Reactions Unit 10.1 Working with Enzymes David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp. Unit 10.2 Overview of PCR Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute Unit 10.3 Real-Time PCR Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College