Pharmaceutical Bioassays

The definitive compendium of bioassay procedures and applications A virtual encyclopedia of key bioassay protocols, this up-to-date, essential resource reviews the methods and applications of bioassays that quantify drug activity and evaluate the validity of pharmacological models. Demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules, the book covers the evaluation and screening of drug compounds in a wide spectrum of therapeutic categories. Throughout, the authors use various models to link experimental observations with findings that clarify the effect of drug compounds on cellular biology and measure the pharmacological activity of chemical substances. This provides an important technological platform for successful drug research and clinical therapy, making Pharmaceutical Bioassays an ideal sourcebook for pharmacologists, pharmaceutical and medical researchers, analytical chemists, toxicologists, and students. Just as a fundamental knowledge of pharmaceutical bioassays is vital to understanding how bioactive agents and new drugs are defined, discovered, and developed, so too is this indispensable reference, which: Offers a complete reference on pharmaceutical bioassays and their specific applications in the assessment of therapies for cancer, Alzheimer’s, diabetes, epilepsy, inflammation, antimalarials, pharmacogenomics, and other major therapeutic areas Covers bioassays that measure toxicity, including those for chemical toxicity and hepatoxicity—a particularly significant consideration in the development of safe and tolerable medications Highlights clinically relevant methods, ideas, and techniques to support any ­pharmaceutical researcher Suggests guidelines and systems to enhance communication between ­pharmacologists, chemists, and biologists Provides an informative analysis of pharmaceutical bioassay technologies and lab applications

Preface xxi Contributors xxiii 1 Methods and Applications of Anticancer Bioassays 1 Shiqi Peng 2 Methods and Applications of Antiviral Assays 23 Shiqi Peng, Ming Zhao, and Chunying Cui 3 Methods and Applications of Antitubercular Assays 39 Shiqi Peng, Ming Zhao, and Chunying Cui 4 Methods and Applications of Thrombus-Related Assays 45 Ming Zhao 5 Methods and Applications of Anticoagulation Assays 65 Ming Zhao 6 Methods and Applications of Blood Pressure-Related Assays 87 Ming Zhao 7 Methods and Applications of Assays Related to Parkinson’s Disease and Graves’ Disease 101 Shiqi Peng 8 Methods and Applications of Alzheimer’s Disease Assays 125 Shiqi Peng 9 Methods and Applications of Antiosteoporosis Assays 145 Ming Zhao 10 Methods and Applications of Immunomodulating Assays 157 Shiqi Peng 11 Methods and Applications of Anti-Inflammatory Assays 187 Ming Zhao 12 Methods and Applications of Antioxidant Activity Assays 205 Shiqi Peng 13 Methods and Applications of Analgesic Assays 223 Ming Zhao 14 Methods and Applications of Epilepsy Assays 237 Ming Zhao, Shiqi Peng, and Guohui Cui 15 Methods and Applications of Diabetes Assays 251 Ming Zhao, Shiqi Peng, and Guohui Cui 16 Methods and Applications of Assays for Toxins from Microorganisms 259 Shiqi Peng 17 Methods and Applications of Toxicity Assays for Chemicals 281 Shiqi Peng 18 Methods and Applications of Hepatoxicity and Hepatoprotective Assays 325 Shiqi Peng 19 Methods and Applications of Estrogen Assays 373 Shiqi Peng 20 Methods and Applications of Antimalarial Assays 397 Shiqi Peng 21 Methods and Applications of Cytogenetic Receptor and Enzyme Assays 419 Shiqi Peng Index 445