Genetic Techniques for Biological Research

Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.

Introduction. SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM 1. Saccharomyces cerevisiae As a Genetic Model Organism 2. Techniques in Cell and Molecular Biology 3. Saccharomyces Cell Structure SECTION II: TECHNIQUES OF GENETIC ANALYSIS 4. Mutant Hunts - To select or to screen (perhaps even by brute force) 5. Complementation analysis: How many genes are involved? 6. Epistasis analysis 7. Gene isolation and analysis of multiple mutant alleles 8. Suppression Analysis 9. Enhancement and synthetic phenotypes 10. Two-hybrid analysis 11. Advanced concepts in molecular genetic analysis 12. Genomic Analysis SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE Case Study I: Glucose Sensing and Signaling mechanisms in Saccharomyces Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces Case Study III: The Cell Division Cycle of Saccharomyces Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces Index.