Cell and Tissue Culture for Medical Research

Cell and tissue culture is a technique in which plant or animal cells are grown under controlled conditions in the laboratory. This is then used for the analysis of the cells themselves, the assessment of the cell's response to chemicals, or as a tool to produce cellular-derived protein products. This book is a collection of fundamental and specific applied procedures in cell and tissue culture which form the basis of the new medical techniques of tissue engineering and gene therapy. It combines both detailed laboratory procedures and informative overviews. * Provides step-by-step protocols with troubleshooting tips and notes on time considerations. * Main procedures are supplemented by alternative procedures, background information and references. * Experimental examples indicate expected results.

Contributors xv Preface xxiii Safety xxiv Chapter 1 Basic Laboratory Set-Up and Procedures 1 Chapter 2 Specialized Techniques 45 Chapter 3 Cell Characterization and Analysis 91 Chapter 4 Cell Line Derivation Methods 147 Chapter 5 Modern Clinical Applications of Cultured Cells 329 Appendix 1: Terminology 425 Appendix 2: Company Addresses 434 Index 439