Flow Cytometry

A VALUABLE RESOURCE FOR INTRODUCING FLOW CYTOMETRY, PROVIDING A FOUNDATION FOR UNDERSTANDING ITS IMPORTANCE IN VARIOUS FIELDS The newly updated and revised Flow Cytometry: First Principles, Third Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting to cell function, plant biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature. There is an extensive glossary at the end of the book. Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on: How flow cytometry is used to analyze cells How a flow cytometer can be used to separate different types of cells from each other Fluidics, electronics, and optics (including lasers, fluorochromes, and filters) Clinical applications of flow cytometry An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students.

The Past as Prologue Setting the Scene Instrumentation: Into the Black Box Information: Harnessing the Data Seeing the Light: Lasers, Fluorochromes, Mirrors, Prisms, and Filters Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating Cells from Within: Intracellular Proteins Cells from Within: DNA in Life and Death Assays of Function The Sorting of Cells Disease and Diagnosis: The Clinical Laboratory Out of the Mainstream: Biological Frontiers Out of the Mainstream" Technological Frontiers Flowing On: The Future Selected General Resources Glossary