Flow Cytometry Principles for Clinical Laboratory Practice

FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE Quality Assurance for Quantitative Immunophenotyping Marilyn A. Owens and Michael R. Loken Flow Cytometry Principles for Clinical Laboratory Practice is aninvaluable benchtop reference and introduction for first-time usersof flow cytometry in clinical diagnostic laboratories. This manualprovides authoritative coverage of accepted methods, qualitycontrols, and standards for the cytometric analysis of clinicalsamples with particular relevance to CD4+ lymphocytes. CD4+lymphocyte determination by immunophenotyping is the standardmethod for evaluating the status of HIV infection, and the singlemost frequent application of flow cytometry in a diagnosticsetting. Flow Cytometry Principles for Clinical Laboratory Practice willappeal to clinical laboratory technologists, medical practitioners,and cytometry lab directors in their role as instructors of newpersonnel. In addition, it is a valuable resource for clinical andbiomedical researchers, establishing quantitative cytometricsystems for studies in immunology and hematology.

Assumptions in Flow Cytometry. Components of Flow Cytometric Immunophenotyping. Quality Control of Flow Cytometer and Reagents. Quantitative Immunophenotyping in HIV Infection. Peripheral Blood Stem Cell Quantitation. Variability in Immunophenotyping. Safety and Specimen Acquisition, Handling, and Processing. Analysis of FCI Results and Troubleshooting. New Methods for Cell Enumeration. Index.