Culture of Human Tumor Cells

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.

Preface vii Contributors ix List of Abbreviations xiii 1. Growth of Human Lung Tumor Cells in Culture 1 Reen Wu 2. Culture of Normal and Malignant Gastric Epithelium 23 Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and MichaelJ.Rutten 3. Establishment of Cell Lines from Colon Carcinoma 67 Robert H. Whitehead 4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application t o an Experimental Model of Pancreatic Cancer Metastasis 81 Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi 5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro 97 Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard 6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells 125 Robert K. Bright and Jennifer D. Lewis 7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites 145 Anne P.Wilson 8. Culture of Cervical Carcinoma Tumor Cell Lines 179 Peter Stern, Catherine West and Deborah Burt 9. Primary Culture of Human Mammary Tumor Cells 205 Valerie Speirs 10. Myoepithelium: Methods of Culture and Study 221 Sanford H. Barsky and Mary L. Alpaugh 11. Multistage Head and Neck Squamous Cell Carcinoma 261 Kirsten G. Edington, lsabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, Gerry Robertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson 12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions 289 Ruth Halaban 13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines 319 Hans G. Drexler 14. In Vitro Culture of Malignant Brain Tumors 349 John L. Darling 15. Culture of Human Neuroendocrine Tumor Cells 373 Roswitha Pfragner, Annemarie Behmel, Elisabeth lngolic and Gerhard H. Wirnsberger Suppliers 405 Index 419