Culture of Hematopoietic Cells

Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Partial table of contents: PRIMITIVE PROGENITOR ASSAYS. Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher). Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa). Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio). COLONY-FORMING ASSAYS FOR MALIGNANT CELLS. Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.). LONG-TERM ASSAYS AND IMMORTALIZATION. Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves). Immortalization of Hematopoietic Cells (C. MacDonald). PURIFICATION OF PROGENITOR CELLS. Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer). OTHER SYSTEMS AND ASSAYS. Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman). List of Suppliers. Index.