Enzyme Kinetics

Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.

Preface. Tools and Techniques of Kinetic Analysis. How do Enzymes Work? Characterization of Enzyme Activity. Reversible Enzyme Inhibition. Irreversible Enzyme Inhibition. pH Dependence of Enzyme-Catalyzed Reactions. Two-Substrate Reactions. Multisite and Cooperative Enzymes. Immobilized Enzymes. Interfacial Enzymes. Transient Phases of Enzymatic Reactions. Characterization of Enzyme Stability. Mechanism-Based Inhibition (Leslie J. Copp). Putting Kinetic Principles into Practice (Kirk L. Parkin). Use of Enzyme Kinetic Data in the Study of Structure-Function Relationships of Proteins (Takuji Tanaka and Rickey Y. Yada). Bibliography. Index.