Metabolomics by In Vivo NMR

Metabolism By In Vivo NMR reviews and extends the experimental and theoretical reports concerning in vivo NMR, a pioneering approach that offers versatile new ways of studying metabolic pathways. Perfectly timed to coincide with recent findings that demonstrate the novelty and strength of this approach, the book covers recent applications in biochemistry, medicine, and psychology; in vivo NMR techniques; MCA; glucose metabolism focusing on glycogen; the value of muscle glycogen measurements in exercise; and much more. This book is unique in linking in vivo 13C NMR measurements of neuronal activity and energetics with applications to functional imaging and certain disease states Provides a fundamental neurochemical explanation of brain activity applicable to functional imaging, theories of neuronal activity and disease states, e.g. epilepsy, psychiatric diseases and developmental disorders Novel and potentially controversial Will inspire future research directions.

Contributors xi Foreword xiii Sir George K. Radda, Medical Research Council, UK Section A: Background 1 Introduction 1 Robert G. Shulman 2 In Vivo NMR Spectroscopy – Techniques; Direct Detection; MRS; Kinetics and Labels; Fluxes; Concentrations 7 Robin de Graaf 3 Metabolic Control Analysis for the NMR Spectroscopist 31 David A. Fell Section B: 13C and 31P NMR Studies of the Regulation of Glucose Metabolism 4 MRS Studies of the Role of the Muscle Glycogen Synthesis Pathway in the Pathophysiology of Type 2 Diabetes 45 Gerald I. Shulman and Douglas L. Rothman 5 Phosphorylation of Allosteric Enzymes can serve Homeostasis rather than Control Flux: the Example of Glycogen Synthase 59 James R.A. Schafer, David A. Fell, Douglas Rothman and Robert G. Shulman 6 Regulation of Glycogen Metabolism in Muscle during Exercise 73 Thomas B. Price 7 13C NMR Studies of Heart Glycogen Metabolism 87 Maren R. Laughlin, Douglas L. Rothman and Robert G. Shulman 8 Bioenergetics Implication of Metabolic Fluctuation during Muscle Contraction 103 Thomas Jue 9 Lactate, Glycogen and Fatigue 125 Robert G. Shulman and Douglas L. Rothman 10 Futile Cycling in Yeast: How to Control Gluttony in the Midst of Plenty 137 Jan den Hollander and Robert G. Shulman 11 Trehalose Energetics in Yeast Spores 149 Robert G. Shulman and Jan den Hollander 12 Metabolic Networks in the Liver by 2H and 13C NMR 159 A. Dean Sherry and Craig R. Malloy 13 Summarized Reflections on Metabolism 175 Robert G. Shulman and James R. A. Schafer Index 185